J pineal Res Мелатонин как иммунорегулирующий агент
Psychoneuroendocrine modulation of regulatory T lymphocyte system: in vivo and in vitro effects of the pineal immunomodulating hormone melatonin
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- Бұл бет үшін навигация:
- MATERIALS AND METHODS
- CONCLUSION
- Extended exposure to dietary melatonin reduces tumor number and size in aged male mice.
Psychoneuroendocrine modulation of regulatory T lymphocyte system: in vivo and in vitro effects of the pineal immunomodulating hormone melatonin.Vigoré L, Messina G, Brivio F, Fumagalli L, Rovelli F, DI Fede G, Lissoni P. BACKGROUND:At present, it is known that cancer-related immunosuppression would mainly depend on an immunosuppressive action mediated by a subtype of CD4+ lymphocytes, the so-called regulatory T lymphocytes (T-reg), which are identified as CD4+CD25+ cells. Moreover, it has been shown that anticancer immunity is under psychoneuroendocrine regulation, mainly mediated by the pineal hormone melatonin (MLT). This study was performed to investigate the in vivo and in vitro effects of MLT on T-reg generation. MATERIALS AND METHODS:We evaluated the in vivo effects of MLT (20 mg/daily orally in the evening) in 20 patients with untreatable metastatic solid tumor and the in vitro effects of MLT incubation (at 10 and 100 pg/ml) of pure lymphocyte cultures on T-reg cell count. RESULTS:MLT induced a statistically significant decline in mean T-reg cell numbers in patients who achieved disease control, whereas no effect was seen in those who had progressed. In contrast, no in vitro effect of MLT incubation was apparent. CONCLUSION:This preliminary study would suggest that MLT may exert in vivo an inhibitory action on T-reg cell generation in cancer patients which is associated with a control of the neoplastic progression, whereas no direct effect was seen in vitro on lymphocyte differentiation. This finding would suggest that MLT may counteract T-reg cell generation in vivo by inhibiting macrophage activity which is involved in stimulating T-reg cell production. Psychoneuroendocrine модуляция регуляторных Т- системы лимфоцитов : в естественных условиях и в пробирке последствий шишковидной иммуномодулирующее гормона мелатонина . VIGORE L , Мессина G , Brivio F , Fumagalli L , Ровелли F , Д. И. Феде G , Лиссони П. редпосылки : В настоящее время известно, что связанных с раком иммуносупрессии будет в основном зависеть от иммуносупрессивной действия , опосредованного подтип CD4 + лимфоцитов , так называемых регуляторных Т- лимфоцитов ( T -REG ), которые определены как CD25 +-клеток CD4 + . Кроме того, было показано, что противоопухолевый иммунитет при psychoneuroendocrine регулирования, главным образом , опосредованного шишковидной гормона мелатонина ( MLT ) . Это исследование было выполнено для расследования в естественных условиях и в пробирке эффектов МЛТ на поколения Т- Изотерм. Материалы и методы: Мы оценили Vivo эффекты в из MLT (20 мг / день перорально вечером) у 20 пациентов с неизлечимой метастатической солидной опухоли и эффектов в пробирке из MLT инкубации (при 10 и 100 пг / мл ) от чистых культур лимфоцитов на Т- рег количество клеток . РЕЗУЛЬТАТЫ: MLT вызывал статистически значимое снижение средних чисел Т- рег клеток у пациентов , которые достигли борьбы с болезнями , в то время как эффект не был замечен в тех, кто прогрессировал . В противоположность этому, никакого эффекта в пробирке MLT инкубации не было очевидным. ЗАКЛЮЧЕНИЕ : Это предварительное исследование позволяет предположить, что MLT может оказывать в естественных условиях тормозящее действие на поколения Т- Изотерм клеток у больных раком , который , связанных с контролем опухолевой прогрессии, в то время как никакого прямого эффекта не наблюдалось в пробирке на лимфоцитов дифференциации . Это открытие предполагает, что MLT может противодействовать поколения Т- клеток рег в естественных условиях путем ингибирования активность макрофагов который участвует в стимулировании производства Т- клеток рег . Exp Gerontol. 2011 Jan;46(1):18-22. doi: 10.1016/j.exger.2010.09.004. Epub 2010 Sep 16. Extended exposure to dietary melatonin reduces tumor number and size in aged male mice.Sharman EH, Sharman KG, Bondy SC. Several sets of male mice were given dietary melatonin over a series of experiments performed during a nine year period. Overall, melatonin-supplemented mice aged ≥26 months at sacrifice had significantly fewer tumors with lower severity than similarly aged control animals. The studies were originally designed to explore the potential of this agent for reducing the rate of onset of some genetic indices of brain aging. When these animals were sacrificed they were routinely examined for overt evidence of tumors and when these were found, a note was made of their occurrence, and of their size. Tumors are commonly found during senescence of several strains of mice. Since tumorigenesis was not the original intent of the study, these observations were recorded but not pursued in greater detail. In this report, these data have now been collated and summarized. This analysis has the disadvantage that tumor origin and morphology were not recorded. However, the study also has the advantage of being conducted over an extended period of time with many groups of animals. In consequence, many extraneous factors, which could be potential confounders, such as seasonal or dietary variations, are unlikely to have interfered with the analysis. The use of more than one mouse strain strengthens the possibility that the findings may have general relevance. Both aged and young animals were included in the original experiments but the tumor incidence in animals younger than 25 months was very low. Длительное воздействие диетического мелатонина уменьшает количество опухоли и размер в возрасте самцов мышей .
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