Методическое пособие и описание лабораторной работы (препринт) Составители: д т. н. Кирьянов К. Г. аспирант Семенчуков И. В
LOCUS HUMMTCG 16569 bp ds-DNA Circular PRI 19-APR-1991
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LOCUS HUMMTCG 16569 bp ds-DNA Circular PRI 19-APR-1991DEFINITION Human mitochondrion, complete genome. ACCESSION J01415 M12548 M58503 M63932 M63933KEYWORDS 12S ribosomal RNA; 16S ribosomal RNA; ATPase; circular; complete genome; cytochrome; cytochrome oxidase; displacement loop; oxidase; ribosomal RNA; transfer RNA; transfer RNA-Ala; transfer RNA-Arg; transfer RNA-Asn; transfer RNA-Asp; transfer RNA-Cys; transfer RNA-Gln; transfer RNA-Glu; transfer RNA-Gly; transfer RNA-His; transfer RNA-Ile; transfer RNA-Leu; transfer RNA-Lys; transfer RNA-Met; transfer RNA-Phe; transfer RNA-Pro; transfer RNA-Ser; transfer RNA-Thr; transfer RNA-Trp; transfer RNA-Tyr; transfer RNA-Val; unidentified reading frame. SOURCE Human placenta mitochondrial DNA; HeLa cells mtDNA; HeLa cells mt mRNA; KB cells mtDNA (see comment). ORGANISM Mitochondrion Homo sapiens Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 105 to 323) AUTHORS Crews,S., Ojala,D., Posakony,J., Nishiguchi,J. and Attardi,G. TITLE Nucleotide sequence of a region of human mitochondrial DNA containing the precisely identified origin of replication JOURNAL Nature 277, 192-198 (1979) STANDARD full automatic REFERENCE 2 (bases 1 to 16569) AUTHORS Anderson,S., Bankier,A.T., Barrell,B.G., de Bruijn,M.H.,
TITLE Sequence and organization of the human mitochondrial genome JOURNAL Nature 290, 457-465 (1981) STANDARD full automatic REFERENCE 3 (bases 1 to 16569) AUTHORS Montoya,J., Ojala,D. and Attardi,G. TITLE Distinctive features of the 5'-terminal sequences of the human
JOURNAL Nature 290, 465-470 (1981) STANDARD full automaticREFERENCE 4 (bases 16015 to 16214) AUTHORS Simmons,J.N., Wright,C.T. and Clayton,D.A. TITLE Elongation of displacement-loop strands in human and mouse
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 6116-6120 (1981) STANDARD full automaticREFERENCE 5 (bases 324 to 743) AUTHORS Bogenhagen,D.F., Applegate,E.F. and Yoza,B.K. TITLE Identification of a promoter for transcription of the heavy strand
JOURNAL Cell 36, 1105-1113 (1984) STANDARD full automatic REFERENCE 6 (bases 15888 to 15954) AUTHORS Mita,S., Monnat,R.J.Jr. and Loeb,L.A. TITLE Direct selection of mutations in the human mitochondrial Thr-tRNA gene: Reversion of an 'unclonable' phenotype JOURNAL Mutat. Res. 199, 183-190 (1988) STANDARD full automatic REFERENCE 7 (bases 1 to 16569) AUTHORS Johns,D.R. and Cornblath,D.R. TITLE Molecular insight into the asymmetric distribution of pathogenetic human mitochondrial DNA deletions JOURNAL Biochem. Biophys. Res. Commun. 174, 244-250 (1991) STANDARD full automatic REFERENCE 8 (sites) AUTHORS Rotig,A., Cormier,V., Koll,F., Mize,C., Saudubray,J.-M.M.,
TITLE Site-specific deletions of the mitochondrial genome in Pearson's marrow-pancreas syndrome JOURNAL Unpublished (1991) STANDARD full automatic REFERENCE 9 (sites) AUTHORS Moraes,C.T., Andreetta,F., Bonilla,E., Shanske,S., DiMauro,S. and Schon,E.A. TITLE Replication-competent human mitochondrial DNA lacking the heavy-strand promoter region JOURNAL Mol. Cell. Biol. 11, 1631-1637 (1991) STANDARD full automatic COMMENT [1] replication initiation site.
FEATURES Location/Qualifiers D-loop complement(join(16104..16569,1..191)) /note="origin H-strand replication (D-loop)" rep_origin 1..191 misc_feature 471..483 repeat_unit 471..478 /note="5' first direct repeat of the deletion junction" repeat_unit 480..483 /note="5' second direct repeat of the deletion junction" misc_signal 531..568 /note="H-strand transcription promoter [5]" mRNA 556..>556 /note="H-strand mRNA [5]" mutation 573..4415 /citation=[9] /note="3843 bp deletion causing external ophthalmoplegia" tRNA 577..647 /anticodon=(pos:611..613,aa:Phe) /note="Phe-tRNA (NAR: 1455)" rRNA 648..1601 /note="12S rRNA" tRNA 1602..1670 /anticodon=(pos:1633..1635,aa:Val) /note="Val-tRNA (NAR: 2053)" rRNA 1671..3229 /note="16S rRNA" tRNA 3230..3304 /anticodon=(pos:3265,aa:Leu) /product="transfer RNA-Leu" /gene="tRNA-Leu" /note="NAR: 1054" mRNA 3305..4262 /note="RNA 13 mRNA" tRNA 4263..4331 /anticodon=(pos:4292..4294,aa:Ile) /note="Ile-tRNA (NAR: 0997)" tRNA complement(4329..4400) /anticodon=(pos:4365..4367,aa:Gln) /note="Gln-tRNA (NAR: 0597)" tRNA 4402..4469 /anticodon=(pos:4432..4434,aa:Met) /note="Met-tRNA-f (NAR: 1297)" mRNA 4470..5511 /note="RNA 12 mRNA" misc_feature 5152..5163 repeat_unit 5152..5159 /note="3' first direct repeat of the deletion junction" repeat_unit 5160..5163 /note="3' second direct repeat of the deletion junction" tRNA 5512..5579 /anticodon=(pos:5544..5546,aa:Trp) /note="Trp-tRNA (NAR: 1897)" tRNA complement(5587..5655) /anticodon=(pos:5623..5625,aa:Ala) /note="Ala-tRNA (NAR: 0097)" tRNA complement(5657..5729) /anticodon=(pos:5694..5696,aa:Asn) /note="Asn-tRNA (NAR: 0297)" rep_origin 5741..5752 /note="(contains) origin L-strand replication" tRNA complement(5761..5826) /anticodon=(pos:5796..5798,aa:Cys) /note="Cys-tRNA (NAR: 0497)" tRNA complement(5826..5891) /anticodon=(pos:5860..5862,aa:Tyr) /note="Tyr-tRNA (NAR: 1997)" mRNA 5901..7517 /note="RNA 9 mRNA" tRNA complement(7445..7516) /anticodon=(pos:7482..7484,aa:Ser) /note="Ser-tRNA-ucn (NAR: 1697)" tRNA 7518..7585 /anticodon=(pos:7548..7550,aa:Asp) /note="Asp-tRNA (NAR: 0397)" mRNA 7586..8294 /note="RNA 16 mRNA" tRNA 8295..8364 /anticodon=(pos:8323..8325,aa:Lys) /note="Lys-tRNA (NAR: 1197)" mRNA 8365..9206 /note="RNA 14 mRNA" mutation 8470..13446 /citation=[8] /note="Nucleotides deleted in Pearson marrow-pancreas syndrome" mRNA 9207..9990 /note="RNA 15 mRNA" tRNA 9991..10058 /anticodon=(pos:10021..10023,aa:Gly) /note="Gly-tRNA (NAR: 0797)" mutation 9996..15896 /citation=[8] /note="Nucleotides deleted in Pearson marrow-pancreas syndrome" mRNA 10059..>10404 /note="RNA 17 mRNA" mutation 10191..13752 /citation=[8] /note="Nucleotides deleted in Pearson marrow-pancreas syndrome" tRNA 10405..10469 /anticodon=(pos:10435..10437,aa:Arg) /note="Arg-tRNA (NAR: 0197)" mRNA 10470..12137 /note="RNA 7 mRNA" mutation 10666..14855 /citation=[8] /note="Nucleotides deleted in Pearson marrow-pancreas syndrome" mutation 11233..13979 /citation=[8] /note="Nucleotides deleted in Pearson marrow-pancreas syndrome" tRNA 12138..12206 /anticodon=(pos:12168..12170,aa:His) /note="His-tRNA (NAR: 0897)" tRNA 12207..12265 /anticodon=(pos:12226..12228,aa:Ser) /note="Ser-tRNA-agy (NAR: 1656)" tRNA 12266..12336 /anticodon=(pos:12298..12300,aa:Leu) /note="Leu-tRNA-cun (NAR: 1097)" mRNA 12337..>14148 /note="RNA 5 mRNA" tRNA complement(14674..14742) /anticodon=(pos:14710..14712,aa:Glu) /note="Glu-tRNA (NAR: 0697)" mRNA 14747..15887 /note="RNA 11 mRNA" mutation 15855 /note="g in wt; a in some HeLa cells [3]" mutation 15880 /note="g in wt; c in some HeLa cells" tRNA 15888..15953 /anticodon=(pos:15919..15921,aa:Thr) /note="Thr-tRNA (NAR: 1797)" mutation 15889 /note="a in wt; g in some HeLa cells" mutation 15919..15922 /note="tata in wt; ta in some HeLa cells" mutation 15920 /note="a in wt; g in some HeLa cells" mutation 15924 /note="t in wt; c in some HeLa cells" mutation 15935..15937 /note="ctt in wt; ct in some HeLa cells" mutation 15936 /note="t in wt; c or g in some HeLa cells" mutation 15937 /note="t in wt; c in some HeLa cells" mutation 15938..15940 /note="tac in wt; tc in some HeLa cells" mutation 15938..15942 /note="tacaa in wt; ta in some HeLa cells" tRNA complement(15955..16023) /anticodon=(pos:15990..15992,aa:Pro) /note="Pro-tRNA (NAR: 1597)" rep_origin 16104..16569 misc_signal 16158..16172 /note="D-loop termination signal" CDS 3307..4262 /transl_except=(pos:4261..4262,aa:other) /note="stop" /product="protein 1" /codon_start=1 /translation="MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQTX" CDS 4470..5511 /transl_except=(pos:5511..5511,aa:other) /note="stop" /product="protein 2" /codon_start=1 /translation="INPLAQPVIYSTIFAGTLITALSSHWFFTWVGLEMNMLAFIPVLTKKMNPRSTEAAIKYFLTQATASMILLMAILFNNMLSGQWTMTNTTNQYSSLMIMMAMAMKLGMAPFHFWVPEVTQGTPLTSGLLLLTWQKLAPISIMYQISPSLNVSLLLTLSILSIMAGSWGGLNQTQLRKILAYSSITHMGWMMAVLPYNPNMTILNLTIYIILTTTAFLLLNLNSSTTTLLLSRTWNKLTWLTPLIPSTLLSLGGLPPLTGFLPKWAIIEEFTKNNSLIIPTIMATITLLNLYFYLRLIYSTSITLLPMSNNVKMKWQFEHTKPTPFLPTLIALTTLLLPISPFMLMILX" CDS 5904..7445 /transl_except=(pos:7443..7445,aa:other) /note="stop" /product="cytochrome oxidase subunit 1" /codon_start=1 /translation="MFADRWLFSTNHKDIGTLYLLFGAWAGVLGTALSLLIRAELGQPGNLLGNDHIYNVIVTAHAFVMIFFMVMPIMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSLLLLLASAMVEAGAGTGWTVYPPLAGNYSHPGASVDLTIFSLHLAGVSSILGAINFITTIINMKPPAMTQYQTPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDPAGGGDPILYQHLFWFFGHPEVYILILPGFGMISHIVTYYSGKKEPFGYMGMVWAMMSIGFLGFIVWAHHMFTVGMDVDTRAYFTSATMIIAIPTGVKVFSWLATLHGSNMKWSAAVLWALGFIFLFTVGGLTGIVLANSSLDIVLHDTYYVVAHFHYVLSMGAVFAIMGGFIHWFPLFSGYTLDQTYAKIHFTIMFIGVNLTFFPQHFLGLSGMPRRYSDYPDAYTTWNILSSVGSFISLTAVMLMIFMIWEAFASKRKVLMVEEPSMNLEWLYGCPPPYHTFEEPVYMKSX" CDS 7586..8269 /product="cytochrome oxidase subunit 2" /codon_start=1 /translation="MAHAAQVGLQDATSPIMEELITFHDHALMIIFLICFLVLYALFLTLTTKLTNTNISDAQEMETVWTILPAIILVLIALPSLRILYMTDEVNDPSLTIKSIGHQWYWTYEYTDYGGLIFNSYMLPPLFLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL" CDS 8366..8572 /note="putative" /product="protein A6L" /codon_start=1 /translation="MPQLNTTVWPTMITPMLLTLFLITQLKMLNTNYHLPPSPKPMKMKNYNKPWEPKWTKICSLHSLPPQS" CDS 8527..9209 /transl_except=(pos:9208..9209,aa:other) /note="stop" /product="ATPase 6" /codon_start=1 /translation="MNENLFASFIAPTILGLPAAVLIILFPPLLIPTSKYLINNRLIT TQQWLIKLTSKQMMTMHNTKGRTWSLMLVSLIIFIATTNLLGLLPHSFTPTTQLSMNLAMAIPLWAGTVIMGFRSKIKNALAHFLPQGTPTPLIPMLVIIETISLLIQPMALAVRLTANITAGHLLMHLIGSATLAMSTINLPSTLIIFTILILLTILEIAVALIQAYVFTLLVSLYLHDNTLX" CDS 9207..9987 /transl_except=(pos:9987..9987,aa:other) /note="stop" /product="cytochrome oxidase subunit 3" /codon_start=1 /translation="MTHQSHAYHMVKPSPWPLTGALSALLMTSGLAMWFHFHSMTLLMLGLLTNTLTMYQWWRDVTRESTYQGHHTPPVQKGLRYGMILFITSEVFFFAGFFWAFYHSSLAPTPQLGGHWPRTGITPLNPLEVPLLNTSVLLASGVSITWAHHSLMENNRNQMIQALLITILLGLYFTLLQASEYFESPFTISDGIYGSTFFVATGFHGLHVIIGSTFLTICFIRQLMFHFTSKHHFGFEAAAWYWHFVDVVWLFLYVSIYWWGX" CDS 10059..10404 /transl_except=(pos:10404..10404,aa:other) /note="stop" /product="protein 3" /codon_start=1 /translation="MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSLAYEWLQKGLDWTEX" CDS 10470..10766 /note="putative" /product="protein 4L" /codon_start=1 /translation="MPLIYMNIMLAFTISLLGMLVYRSHLMSSLLCLEGMMLSLFIMATLMTLNTHSLLANIVPIAMLVFAACEAAVGLALLVSISNTYGLDYVHNLNLLQC" CDS 10760..12137 /transl_except=(pos:12137..12137,aa:other) /note="stop" /product="protein 4" /codon_start=1 /translation="MLKLIVPTIMLLPLTWLSKKHMIWINTTTHSLIISIIPLLFFNQINNNLFSCSPTFSSDPLTTPLLMLTTWLLPLTIMASQRHLSSEPLSRKKLYLSMLISLQISLIMTFTATELIMFYIFFETTLIPTLAIITRWGNQPERLNAGTYFLFYTLVGSLPLLIALIYTHNTLGSLNILLLTLTAQELSNSWANNLMWLAYTMAFMVKMPLYGLHLWLPKAHVEAPIAGSMVLAAVLLKLGGYGMMRLTLILNPLTKHMAYPFLVLSLWGMIMTSSICLRQTDLKSLIAYSSISHMALVVTAILIQTPWSFTGAVILMIAHGLTSSLLFCLANSNYERTHSRIMILSQGLQTLLPLMAFWWLLASLANLALPPTINLLGELSVLVTTFSWSNITLLLTGLNMLVTALYSLYMFTTTQWGSLTHHINNMKPSFTRENTLMFMHLSPILLLSLNPDIITGFSSX" CDS 12337..14148 /note="putative" /product="protein 5" /codon_start=1 /translation="MTMHTTMTTLTLTSLIPPILTTLVNPNKKNSYPHYVKSIVASTFIISLFPTTMFMCLDQEVIISNWHWATTQTTQLSLSFKLDYFSMMFIPVALFVTWSIMEFSLWYMNSDPNINQFFKYLLIFLITMLILVTANNLFQLFIGWEGVGIMSFLLISWWYARADANTAAIQAILYNRIGDIGFILALAWFILHSNSWDPQQMALLNANPSLTPLLGLLLAAAGKSAQLGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLIRFHPLAENSPLIQTLTLCLGAITTLFAAVCALTQNDIKKIVAFSTSSQLGLMMVTIGINQPHLAFLHICTHAFFKAMLFMCSGSIIHNLNNEQDIRKMGGLLKTMPLTSTSLTIGSLALAGMPFLTGFYSKDHIIETANMSYTNAWALSITLIATSLTSAYSTRMILLTLTGQPRFPTLTNINENNPTLLNPIKGLAAGSLFAGFLITNNISPASPFQTTIPLYLKLTALAVTFLGLLTALDLNYLTNKLKMKSPLCTFYFSNMLGFYPSITHRTIPYLGLLTSQNLPLLLLDLTWLEKLLPKTISQHQISTSIITSTQKGMIKLYFLSFFFPLILTLLLIT" CDS complement(14148..14673) /transl_except=(pos:14673..14673,aa:other) /note="stop" /product="protein 6" /codon_start=1 /translation="MMYALFLLSVGLVMGFVGFSSKPSPIYGGLVLIVSGVVGCVIILNFGGGYMGLMVFLIYLGGMMVVFGYTTAMAIEEYPEAWGSGVEVLVSVLVGLAMEVGFVLWVKEYDGVVVVVNFNSVGSWMIYEGEGSGFIREDPIGAGALYDYGRWLVVVTGWPLFVGVYIVIEIARGNRX" CDS 14747..15881 /transl_except=(pos:15881..15881,aa:other) /note="stop" /product="cytochrome b" /codon_start=1 /translation="MTPMRKINPLMKLINHSFIDLPTPSNISAWWNFGSLLGACLILQITTGLFLAMHYSPDASTAFSSIAHITRDVNYGWIIRYLHANGASMFFICLFLHIGRGLYYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFLHETGSNNPLGITSHSDKITFHPYYTIKDALGLLLFLLSLMTLTLFSPDLLGDPDNYTLANPLNTPPHIKPEWYFLFAYTILRSVPNKLGGVLALLLSILILAMIPILHMSKQQSMMFRPLSQSLYWLLAADLLILTWIGGQPVSYPFTIIGQVASVLYFTTILILMPTISLIENKMLKX" BASE COUNT 5123 a 5175 c 2177 g 4094 t ORIGIN MboI site in D-loop (H-strand replication) region 1 gatcacaggt ctatcaccct attaaccact cacgggagct ctccatgcat ttggtatttt 61 cgtctggggg gtatgcacgc gatagcattg cgagacgctg gagccggagc accctatgtc 121 gcagtatctg tctttgattc ctgcctcatc ctattattta tcgcacctac gttcaatatt 181 acaggcgaac atacttacta aagtgtgtta attaattaat gcttgtagga cataataata 241 acaattgaat gtctgcacag ccactttcca cacagacatc ataacaaaaa atttccacca 301 aaccccccct cccccgcttc tggccacagc acttaaacac atctctgcca aaccccaaaa 361 acaaagaacc ctaacaccag cctaaccaga tttcaaattt tatcttttgg cggtatgcac 421 ttttaacagt caccccccaa ctaacacatt attttcccct cccactccca tactactaat 481 ctcatcaata caacccccgc ccatcctacc cagcacacac acaccgctgc taaccccata 581 ccccgaacca accaaacccc aaagacaccc cccacagttt atgtagctta cctcctcaaa 601 gcaatacact gaaaatgttt agacgggctc acatcacccc ataaacaaat aggtttggtc 661 ctagcctttc tattagctct tagtaagatt acacatgcaa gcatccccgt tccagtgagt 721 tcaccctcta aatcaccacg atcaaaagga acaagcatca agcacgcagc aatgcagctc 781 aaaacgctta gcctagccac acccccacgg gaaacagcag tgattaacct ttagcaataa 841 acgaaagttt aactaagcta tactaacccc agggttggtc aatttcgtgc cagccaccgc 901 ggtcacacga ttaacccaag tcaatagaag ccggcgtaaa gagtgtttta gatcaccccc 961 tccccaataa agctaaaact cacctgagtt gtaaaaaact ccagttgaca caaaatagac 1021 tacgaaagtg gctttaacat atctgaacac acaatagcta agacccaaac tgggattaga 1081 taccccacta tgcttagccc taaacctcaa cagttaaatc aacaaaactg ctcgccagaa 1141 cactacgagc cacagcttaa aactcaaagg acctggcggt gcttcatatc cctctagagg 1201 agcctgttct gtaatcgata aaccccgatc aacctcacca cctcttgctc agcctatata 1261 ccgccatctt cagcaaaccc tgatgaaggc tacaaagtaa gcgcaagtac ccacgtaaag 1321 acgttaggtc aaggtgtagc ccatgaggtg gcaagaaatg ggctacattt tctaccccag 1381 aaaactacga tagcccttat gaaacttaag ggtcgaaggt ggatttagca gtaaactaag 1441 agtagagtgc ttagttgaac agggccctga agcgcgtaca caccgcccgt caccctcctc 1501 aagtatactt caaaggacat ttaactaaaa cccctacgca tttatataga ggagacaagt 1561 cgtaacatgg taagtgtact ggaaagtgca cttggacgaa ccagagtgta gcttaacaca 1621 aagcacccaa cttacactta ggagatttca acttaacttg accgctctga gctaaaccta 1681 gccccaaacc cactccacct tactaccaga caaccttagc caaaccattt acccaaataa 1741 agtataggcg atagaaattg aaacctggcg caatagatat agtaccgcaa gggaaagatg 1801 aaaaattata accaagcata atatagcaag gactaacccc tataccttct gcataatgaa 1861 ttaactagaa ataactttgc aaggagagcc aaagctaaga cccccgaaac cagacgagct 1921 acctaagaac agctaaaaga gcacacccgt ctatgtagca aaatagtggg aagatttata 1981 ggtagaggcg acaaacctac cgagcctggt gatagctggt tgtccaagat agaatcttag 2041 ttcaacttta aatttgccca cagaaccctc taaatcccct tgtaaattta actgttagtc 2101 caaagaggaa cagctctttg gacactagga aaaaaccttg tagagagagt aaaaaattta 2161 acacccatag taggcctaaa agcagccacc aattaagaaa gcgttcaagc tcaacaccca 2221 ctacctaaaa aatcccaaac atataactga actcctcaca cccaattgga ccaatctatc 2281 accctataga agaactaatg ttagtataag taacatgaaa acattctcct ccgcataagc 2341 ctgcgtcaga ttaaaacact gaactgacaa ttaacagccc aatatctaca atcaaccaac 2401 aagtcattat taccctcact gtcaacccaa cacaggcatg ctcataagga aaggttaaaa 2461 aaagtaaaag gaactcggca aatcttaccc cgcctgttta ccaaaaacat cacctctagc 2521 atcaccagta ttagaggcac cgcctgccca gtgacacatg tttaacggcc gcggtaccct 2581 aaccgtgcaa aggtagcata atcacttgtt ccttaaatag ggacctgtat gaatggctcc 2641 acgagggttc agctgtctct tacttttaac cagtgaaatt gacctgcccg tgaagaggcg 2701 ggcataacac agcaagacga gaagacccta tggagcttta atttattaat gcaaacagta 2761 cctaacaaac ccacaggtcc taaactacca aacctgcatt aaaaatttcg gttggggcga 2821 cctcggagca gaacccaacc tccgagcagt acatgctaag acttcaccag tcaaagcgaa 2881 ctactatact caattgatcc aataacttga ccaacggaac aagttaccct agggataaca 2941 gcgcaatcct attctagagt ccatatcaac aatagggttt acgacctcga tgttggatca 3001 ggacatcccg atggtgcagc cgctattaaa ggttcgtttg ttcaacgatt aaagtcctac 3061 gtgatctgag ttcagaccgg agtaatccag gtcggtttct atctaccttc aaattcctcc 3121 ctgtacgaaa ggacaagaga aataaggcct acttcacaaa gcgccttccc ccgtaaatga 3181 tatcatctca acttagtatt atacccacac ccacccaaga acagggtttg ttaagatggc 3241 agagcccggt aatcgcataa aacttaaaac tttacagtca gaggttcaat tcctcttctt 3301 aacaacatac ccatggccaa cctcctactc ctcattgtac ccattctaat cgcaatggca 3361 ttcctaatgc ttaccgaacg aaaaattcta ggctatatac aactacgcaa aggccccaac 3421 gtggtaggcc cctacgggct actacaaccc ttcgctgacg ccataaaact cttcaccaaa 3481 gagcccctaa aacccgccac atctaccatc accctctaca tcaccgcccc gaccttagct 3541 ctcaccatcg ctcttctact atgaaccccc ctccccatac ccaaccccct ggtcaacctc 3601 aacctaggcc tcctatttat tctagccacc tctagcctag ccgtttactc aatcctctga 3661 tcagggtgag catcaaactc aaactacgcc ctgatcggcg cactgcgagc agtagcccaa 3721 acaatctcat atgaagtcac cctagccatc attctactat caacattact aataagtggc 3781 tcctttaacc tctccaccct tatcacaaca caagaacacc tctgattact cctgccatca 3841 tgacccttgg ccataatatg atttatctcc acactagcag agaccaaccg aacccccttc 3901 gaccttgccg aaggggagtc cgaactagtc tcaggcttca acatcgaata cgccgcaggc 3961 cccttcgccc tattcttcat agccgaatac acaaacatta ttataataaa caccctcacc 4021 actacaatct tcctaggaac aacatatgac gcactctccc ctgaactcta cacaacatat 4081 tttgtcacca agaccctact tctaacctcc ctgttcttat gaattcgaac agcatacccc 4141 cgattccgct acgaccaact catacacctc ctatgaaaaa acttcctacc actcacccta 4201 gcattactta tatgatatgt ctccataccc attacaatct ccagcattcc ccctcaaacc 4261 taagaaatat gtctgataaa agagttactt tgatagagta aataatagga gcttaaaccc 4321 ccttatttct aggactatga gaatcgaacc catccctgag aatccaaaat tctccgtgcc 4381 acctatcaca ccccatccta aagtaaggtc agctaaataa gctatcgggc ccataccccg 4441 aaaatgttgg ttataccctt cccgtactaa ttaatcccct ggcccaaccc gtcatctact 4501 ctaccatctt tgcaggcaca ctcatcacag cgctaagctc gcactgattt tttacctgag 4561 taggcctaga aataaacatg ctagctttta ttccagttct aaccaaaaaa ataaaccctc 4621 gttccacaga agctgccatc aagtatttcc tcacgcaagc aaccgcatcc ataatccttc 4681 taatagctat cctcttcaac aatatactct ccggacaatg aaccataacc aatactacca 4741 atcaatactc atcattaata atcataatag ctatagcaat aaaactagga atagccccct 4801 ttcacttctg agtcccagag gttacccaag gcacccctct gacatccggc ctgcttcttc 4861 tcacatgaca aaaactagcc cccatctcaa tcatatacca aatctctccc tcactaaacg 4921 taagccttct cctcactctc tcaatcttat ccatcatagc aggcagttga ggtggattaa 4981 accagaccca gctacgcaaa atcttagcat actcctcaat tacccacata ggatgaataa 5041 tagcagttct accgtacaac cctaacataa ccattcttaa tttaactatt tatattatcc 5101 taactactac cgcattccta ctactcaact taaactccag caccacgacc ctactactat 5161 ctcgcacctg aaacaagcta acatgactaa cacccttaat tccatccacc ctcctctccc 5221 taggaggcct gcccccgcta accggctttt tgcccaaatg ggccattatc gaagaattca 5281 caaaaaacaa tagcctcatc atccccacca tcatagccac catcaccctc cttaacctct 5341 acttctacct acgcctaatc tactccacct caatcacact actccccata tctaacaacg 5401 taaaaataaa atgacagttt gaacatacaa aacccacccc attcctcccc acactcatcg 5461 cccttaccac gctactccta cctatctccc cttttatact aataatctta tagaaattta 5521 ggttaaatac agaccaagag ccttcaaagc cctcagtaag ttgcaatact taatttctgt 5581 aacagctaag gactgcaaaa ccccactctg catcaactga acgcaaatca gccactttaa 5641 ttaagctaag cccttactag accaatggga cttaaaccca caaacactta gttaacagct 5701 aagcacccta atcaactggc ttcaatctac ttctcccgcc gccgggaaaa aaggcgggag 5761 aagccccggc aggtttgaag ctgcttcttc gaatttgcaa ttcaatatga aaatcacctc 5821 ggagctggta aaaagaggcc taacccctgt ctttagattt acagtccaat gcttcactca 5881 gccattttac ctcaccccca ctgatgttcg ccgaccgttg actattctct acaaaccaca 5941 aagacattgg aacactatac ctattattcg gcgcatgagc tggagtccta ggcacagctc 6001 taagcctcct tattcgagcc gagctgggcc agccaggcaa ccttctaggt aacgaccaca 6061 tctacaacgt tatcgtcaca gcccatgcat ttgtaataat cttcttcata gtaataccca 6121 tcataatcgg aggctttggc aactgactag ttcccctaat aatcggtgcc cccgatatgg 6181 cgtttccccg cataaacaac ataagcttct gactcttacc tccctctctc ctactcctgc 6241 tcgcatctgc tatagtggag gccggagcag gaacaggttg aacagtctac cctcccttag жүктеу/скачать 326.52 Kb. Достарыңызбен бөлісу:
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