С. А. Корниенко А. В. Кривопалов
A NEW CELL LINE DERIVED FROM EXPERIMENTAL
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| A NEW CELL LINE DERIVED FROM EXPERIMENTAL
CHOLANGIOCARCINOMA ASSOCIATED WITH OPISTHORCHIASIS FELINEA Minkova G.A., Shilov A.G., Ponomarev D.V., Lvova M.N., Romanenko S.A., Pakharukova M.Y., Mordvinov V.A. Cholangiocarcinoma (CCA) is one of the most serious complications of opisthorchiasis. It is an aggressive tumor that is difficult to treat and diagnose at an early stage due to lack of specific symptoms and specific tumor markers. Furthermore, because of poor response to drug therapy research for new effective treatment and approach is required. Mechanisms of cholangiocarcinogenesis associated with O. felineus infection are not studied. The aim of the work was establishment of cholangiocarcinoma cell line and determination of CCA biomarkers. A cell line HamCCA-1 was derived from the CCA tissue of O. felineus-infected and dimethylnitrosamine-treated Syrian hamsters Mesocricetus auratus. Tumorigenicity of HamCCA-1 cells was demonstrated by allograft transplantation in hamster. Histological examination of transplanted tumors showed glandular structure similar to the primary CCA in hamster liver. To validate HamCCA-1 cell line, standard and potential tumor markers of human CCA, such as keratin 7, annexin A1, exostosin 1 and vimentin were identified. Immunocytochemistry analysis of the cell culture revealed positive staining of all specific markers. In conclusion, the models can be used for studying cholangiocarcinogenesis, as well as for searching CCA treatments and methods for early diagnosis of the disease. Паразитологические исследования в Сибири и на Дальнем Востоке 92 УДК 595.771:591.5 (571.56) РАСПРОСТРАНЕНИЕ МОКРЕЦОВ (DIPTERA: CERATOPOGONIDAE) В ЯКУТИИ Мирзаева А.Г. жүктеу/скачать 7.14 Mb. Достарыңызбен бөлісу:
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