Methods for analyzing the raw material of

Some literature shows a procedure for the quantifi -

cation of procyanidins in hawthorn fruits using modifi ed 

Porter’s method. According to this method, extraction of 

hawthorn fruits is carried out with 70% ethanol, then an 

acid solution of butanol and an iron reagent (for acidic 

cleavage of procyanidins to anthocyanins) are added to 

the extract. After that the optical density of the solution 

is measured by a spectrophotometer at the wavelength of 

550 nm [41].

Samara scientists proposed a technique for analyz-

ing the contents of the sum of hawthorn fruits fl avonoids 

with preliminary extraction on the basis of 70% ethyl 

alcohol in terms of hyperoside using differential spectro-

photometry at the wavelength of 412 nm [42].

3.2. Flowers

For hawthorn fl owers, a method was proposed for 

quantifying the amount of fl avonoids in terms of hypero-

side by differential spectrophotometry at the wavelength 

of 412 nm and preliminary extraction on the basis of 70% 

ethyl alcohol [43].