Quantifi cation of fl avonoids in hawthorn raw

Extraction is one of the most important parameter 

in the quantitative determination in medicinal plants raw 

materials.

For the purposes of quantitative determination of 

hawthorn raw materials in different Pharmacopoeias, 

various extraction options are used. Thus, for the 

extraction of hawthorn fruits, the State Pharmacopoeia 

of the USSR, XI-th edition, offers heating with 95% 

alcohol for 1 hour, and the European Pharmacopoeia, 

the Pharmacopoeias of the Republic of Belarus and 

the Republic of Kazakhstan, offer 70% ethyl alcohol 

extraction while heating for 30 minutes.

According to the Pharmacopoeia of the Republic 

of Belarus, fl owers are extracted with 96% ethanol 

while heating for 1 hour, and according to the State 

Pharmacopoeia of the USSR, XI-th edition, they are 

extracted with 95% ethanol while heating for 1 hour [28].

According to the US Pharmacopeia, extraction of 

leaves with fl owers is carried out with methanol for 5 

hours, and according to the European and Belarusian 

pharmacopoeias it is done with 60% alcohol for 10 

minutes [27].

According to the Belarusian Pharmacopoeia, leaves 

are extracted with 70% ethyl alcohol while heating for 1 

hour [28].

As far as we can see, the extraction time and extractant 

for the same types of raw materials vary greatly, which 

indicates the necessity for additional studies on selection 

of the optimal extractant and extraction conditions. When 

developing a methodology for quantifying the amount 

of  fl avonoids, it is important to scientifi cally select the 

extractant, taking into account the characteristics of the 

phytochemical composition of raw materials.