Issn 305-9397. Ғылым және білім. 2022. №1-1 (66) issn 2305-9397
ISSN 2305-9397. Ғылым және білім. 2022. № 3-3 (68)
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| ISSN 2305-9397. Ғылым және білім. 2022. № 3-3 (68)____ _
171 them to respond to environmental factors. Accurate identification of phytopathogenic bacteria is important for the diagnosis and prediction of bacterioses, especially for species that are difficult or impossible to cultivate. The proposed study is aimed at developing an express method for identifying phytopathogenic bacterioses of cereal crops (belonging to the genera Pseudomonas and Xanthomonas) based on a fast version of the PCR method - Xtreme Chain Reaction (XCR) and isothermal DNA amplification (LAMP) as an effective platform for identifying bacterial DNA and determining structures of populations and pathogens of black and basal bacterioses [17, 18]. XCR and LAMP are highly specific methods for amplifying the DNA of specific targets, capable of identifying target DNA quickly and highly precisely, which is important in the identification of quarantine infections, various monitoring studies, and customs control of food quality in cases of export or import. Research on the development of a method for identifying bacterioses of cereal crops based on XCR and LAMP methods is new both for Kazakhstan and the world community. The scientific novelty lies in the development of a method for the molecular genetic identification of bacterioses of cereal crops by highly specific methods of extreme polymerase reaction (XCR) and the method of isothermal DNA amplification (LAMP). For the first time, genetic diversity analysis of bacteriosis strains that affect wild cereals and important agricultural cereal crops will be carried out. In connection with Kazakhstan's accession to the WTO and the expansion of trade with the countries of Asia and Africa, the likelihood of the penetration of dangerous bacterial diseases of wheat and other cereals is very high. In this regard, wheat seeds imported from countries where bacteriosis is common must necessarily undergo a phytoexamination and be sown for soil control in a quarantine nursery. жүктеу/скачать 7.12 Mb. Достарыңызбен бөлісу:
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