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172 Table 1 – Composition of the PCR mixture Name of the components of the mixture Volume per reaction Volume for 10 reactions PCR Buffer 1.5 mkl 15 mkl Direct primer 0.5 mkl 5 mkl Reverse primer 0.5 mkl 5 mkl dNTPs 0.5 mkl 5 mkl DNA polymerase (DreamTaq) 0.1 mkl 1 mkl Deionized water (MilliQ) 9.9 mkl 99 mkl Table 2 – Oligonucleotide PCR primers, characterization of PCR products Object(s) of identification Primer ID and sequence Marker Amplicon size (b. p.) Bacteria 2019-31 5'- AGAGTTTGATCCTGGCTCAG 2019-32 5'- TACGGCTACCTTGTTACGACTT 16S 1500 Pseudomonas genera 2019-36 5'- TCGARTGCACSGGBCTSTTCACC 2019-37 5'- GTGTGRTTGGCRTCGAARATCGA gapA 562 2019-40 5'- TCBGCRGCVGARGTSATCATGAC 2019-41 5'- TTGTCYTTGGTCTGSGAGCTGAA gyrB 751 2019-44 5'- ACTGACACTGAGGTGCGAAAGCG 2019-45 5'- ACCGTATGCGCTTCTTCACTTGACC ITS 1322 2019-42 5'- GYGAAGGCGARATYGRAATCG 2019-43 5'- CCGATGTTGCCTTCCTGGATCAG rpoD 858 2019-38 5'- CCTGRTCGCCAAGATGCCGAC 2019-39 5'- CGAAGATCACGGTGAACATGCTGG gltA 665 Xanthomonas genera 2019-17 5'- GGTGGAAGACCTGGTCAAGA 2019-18 5'- TCCTTGACYTCGGTGAACTC dnaK 987 2019-19 5'- AGCTACGAYGTGCGYTACGA 2019-20 5'- GTTCACGCCRAACTGGTAG fyuA 723 2019-21 5'- ACGAGTACAACCCGGACAA 2019-22 5'- CCCATCARGGTGCTGAAGAT gyrB 865 2019-23 5'- TGGAACAGGGCTATCTGACC 2019-24 5'- CATTCYAGGTTGGTCTGRTT rpoD 875 2019-25 5'- TCCTAAGCACCTCAACATCAAG 2019-26 5'- CTTCTTGTCGTCCTTGACCTC dnaK 940 2019-27 5'- CTCGCAGAACGGCCTGTA 2019-28 5'- GTAGCCGGGCATCTTCAACT fyuA 698 2019-29 5'- GTTCCCGGGCCTTGTACACAC 2019-30 5'- GGTTCTTTTCACCTTTCCCTC ITS 1128 2019-33 5'- CGGAATTCTCGGGCAAGACCACC 2019-34 5'- TAGCAAGCTTGTTCTTGACCACCTT recA 564 |