Лекции Reviews, Lectures

conducted with the use of the State Pharmacopoeia of 

the USSR (XI-th edition), the State Pharmacopoeia of 

the Russian Federation (XIII-th edition), the European 

Pharmacopoeia (6-th edition), the United States 

Pharmacopeia (32-nd edition), the State Pharmacopoeia of 

the Republic of Belarus and the Republic of Kazakhstan, 

as well as the information and search (PubMed) and 

library databases (eLibrary).

RESULTS AND DISCUSSION

1. Determination of the authenticity of hawthorn 

raw materials

1.1. Fruits

In the section “Qualitative reactions” for the fruits of 

plants of the genus Hawthorne, the State Pharmacopoeia 

of the USSR (XI-th edition) describes a thin layer 

chromatography (TLC) method, which involves the 

detection of a hyperoside in the presence of a state standard 

sample (SSS) of the hyperoside in the chloroform-

methyl alcohol system (8: 2). Hereby, the detection of the 

analytes is carried out in the ultraviolet light (UV light) 

at the wavelength of 360 nm. At the spot level of the 

hyperoside state standard sample (SSS), a strip of dark 

brown color should appear, then the plate is treated with 

a 5% alcohol solution of aluminum chloride and heated. 

After that the spot acquires a yellow-green fl uorescence 

in the UV light (hyperoside) at the wavelength of 360 

nm [24]. The European Pharmacopoeia (6-th edition), 

the State Pharmacopoeias of the Republic of Belarus 

and the Republic of Kazakhstan describe thin layer 

chromatography on a plate with a layer of silica gel P 

(solvent system: acidic anhydrous water-methyl ethyl 

ketone-ethyl acetate 10: 10: 30: 50, V / V / V / V) using a 

reference solution consisting of chlorogenic acid, caffeic 

acid, hyperoside and rutin dissolved in methanol, as a 

112

Фармация и фармакология Т. 6 № 2, 2018

жүктеу/скачать 488.75 Kb.

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