Лекции Reviews, Lectures

In the US Pharmacopeia, a qualitative determination 

of leaves with hawthorn fl owers is carried out using thin 

layer chromatography. As standards, a solution of rutin, 

chlorogenic acid, hyperoside and vitexin is used. The sol-

vent system includes the following ingredients: ethyl ace-

tate – water – glacial acetic acid – formic acid (10 : 2.6 : 1.1 

: 1.1). The manifestation of the plate is carried out with a 

solution of 2-aminoethyldiphenylborate in methanol (1%), 

then by treatment with a solution of polyethylene glycol 

4000 in methanol (5%) and then viewed in UV light [27].

Besides, the US Pharmacopeia provides a qual-

itative analysis of leaves with hawthorn fl owers  by 

high-performance liquid chromatography (HPLC). The 

reference solution consists of rutin, chlorogenic acid, 

hyperoside and vitexin dissolved in methanol. Solution 

A consists of a mixture of tetrahydrofuran, methanol 

and acetonitrile (92.4 : 4.2 : 3.4). Solution B consists 

of a 0.5% solution of phosphoric acid in water. The de-

tection is carried out at the wavelength of 336 nm on 

a 4.0 mm × 10 cm column that contains 5 μm L1 fi ller 

particles. The column temperature is maintained at 25° 

C. Separately, equal volumes (5 μl) of the test solution 

and the reference solution are introduced into the chro-

matograph, then the retention time of the main peaks is 

measured. For the reference solution, the retention time 

is shown in Table 1.