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method for the qualitative analysis of hawthorn fruits. 

After passing through the solvent front, the plate is dried 

and sprayed with a 1% solution of aminoethyl ester of 

diphenylboric acid in methanol and then with a 50 g / l 

solution of macrogol 400 in methanol. 

Further, the plate is viewed in ultraviolet light 

at the wavelength of 365 nm. As a result of these 

actions the following fl uorescence zones (bottom-

up as Rf increases) should be visible on the plate in 

the case of the reference solution: a yellow-brown 

fl uorescent zone (routine), a light blue fl uorescent zone 

(chlorogenic acid) and a yellowish-brown fl uorescent 

zone (hyperoside); in the upper third of the plate a light 

blue  fl uorescent zone (caffeic acid) should be visible. 

In the case of the test solution, 3 zones are found on 

the plate. They are similar to a reference solution in 

location and fl uorescence, these zones correspond to 

chlorogenic acid, hyperoside and coffee acid. Besides, 

there are 3 weak reddish fl uorescent zones, one of which 

corresponds to rutin according to its location, and the 

other two zones are located above the hyperoside zone 

and below the fl uorescent light blue zone of which is 

below the caffeic acid zone [26, 28, 34].

In our opinion, the above mentioned substances 

used as standard samples (chlorogenic acid, caffeic acid, 

hyperoside and rutin) are widely found in medicinal 

plants and, therefore, are not specifi c for hawthorn fruits. 

Therefore, this approach with regard to the analysis of 

hawthorn fruits appears to be hardly expedient.

Flowers are represented in the State Pharmacopoeia 

of the USSR (XI-th edition) and Pharmacopoeia of the 

Republic of Belarus.

For the qualitative analysis of hawthorn fl owers 

the State Pharmacopoeia of the USSR (XI-th edition) 

suggests the thin layer chromatography (TLC) method 

on “Siloufol” plates in the presence of a standard 

hyperoside sample in the system of chloroform-methyl 

alcohol (8: 2). Hereby the detection is carried out in 

ultraviolet light at the wavelength of 360 nm. At the 

spot level of the standard hyperoside sample a dark 

brown strip should appear. The plate is then treated with 

a 5% alcohol solution of aluminum chloride and heated. 

After that the spot acquires a yellow-green fl uorescence 

in UV light (hyperoside) at the wavelength of 360 nm 

[24].

In the Pharmacopeia of the Republic of Belarus, as a 

thin layer chromatography method is shown in the system 

of chloroform-methanol (80:20, v / v) with the use of 

hyperoside as a standard sample. The manifestation of the 

plate is carried out in ultraviolet light at the wavelength 

of 365 nm in the daylight, pretreating the plate with a 

solution of aluminum chloride followed by heating. In 

ultraviolet light a yellow-green fl uorescence (hyperoside) 

is seen, and in the daylight it is bright yellow [28].

We consider the approach with the use of a standard 

hyperoside sample, which is one of the characteristic 

fl avonoids of hawthorn fl owers, objective, as it makes it 

possible to adequately determine the authenticity of these 

raw materials.