Лекции Reviews, Lectures

Pharmacopoeia and the Pharmacopoeias of the Republic 

of Belarus and the Republic of Kazakhstan presupposes 

a spectrophotometric determination of the quantity of 

procyanidins in terms of cyanidin chloride, which should 

be at least 0.06% (European Pharmacopoeia) and not less 

than 1.0% (Pharmacopoeias of the Republic of Belarus 

and the Republic of Kazakhstan), at the wavelength 

of 550 nm (European Pharmacopoeia) and 545 nm 

(Pharmacopoeias of the Republic of Belarus and the 

Republic of Kazakhstan).

Besides, the Pharmacopoeia of the Republic of 

Belarus describes the method of chromatographic 

spectrophotometric determination of fl avonoids in terms 

of hyperoside, the quantity of which should be at least 

0.06% at the wavelength of 365 nm in dry fruits [26, 28, 

34].

As far as we can see, in the submitted normative 

the quantity of fl avonoids in hawthorn fruits are 

recommended. Besides, there is a difference not only 

in the method of determination (chromatography-

spectrophotometric and spectrophotometric), but also in 

the choice of the quality index (the sum of fl avonoids in 

terms of hyperoside or the sum of procyanidins).

2.2. Flowers

In the State Pharmacopoeia of the USSR, XI-

th edition, a chromatographic-spectrophotometric 

determination of the quantity of hyperoside in fl owers 

(at least 0.5%) at the wavelength of 360 nm is given for 

quantifi cation of hawthorn fl owers [24].

In the Pharmacopoeia of the Republic of Belarus, a 

quantitative analysis of hawthorn fl owers is carried out 

by a chromatography-spectrophotometric method, by 

which the quantity of a hyperoside is determined, it must 

be at least 0.5%, at the wavelength of 365 nm [28].

Chromato-spectrophotometric determination, in our 

opinion, is cumbersome, diffi cult to perform and is not 

deprived of a lack in terms of the accuracy of the method 

[24, 31].