Лекции Reviews, Lectures

In the US Pharmacopeia, quantifi cation of hawthorn 

leaves includes the determination of C-glycosylated 

fl avones in terms of vitexin (the quantity should 

be at least 0.6%), and O-glycosylated fl avones  in 

terms of hyperoside (the quantity should be at least 

0.45%). Hereby the method of high-performance 

liquid chromatography is used. The detection for the 

determination of C-glycosylated fl avons is carried out 

on a 4 mm x 10 cm column at the wavelength of 336 

nm and a fl ow rate of about 1.0 ml per minute. In the 

determination of O-glycosylated fl avons (the term is 

taken from the Pharmacopoeia of the USA, but here it is 

a matter of fl avonols), the detection is carried out on a 4.6 

mm x 25 cm column at the wavelength of 370 nm with a 

fl ow rate of 1.5 ml per minute [27].

Quantifi cation of target substances in leaves with 

fl owers according to the European Pharmacopoeia and 

the Pharmacopoeia of the Republic of Belarus includes 

spectrophotometric determination of the quantity of 

the sum of fl avonoids in terms of hyperoside. In raw 

materials their quantity should be not less than 1.5% at 

the wavelength of 405 nm (European Pharmacopoeia) 

and 410 nm (the Pharmacopoeia of the Republic of 

Belarus) [26, 28].

Apparently, in this case an further investigation is 

necessary for compliance with the choice of the analyti-

cal wavelength characteristic of the substances contain-

ing in raw materials.

2.4. Leaves

In the Pharmacopoeia of the Republic of Belarus, for 

quantifi cation of active substances in hawthorn leaves, 

spectrophotometric determination of the quantity of fl a-

vonoids in terms of rutin (not less than 0.25%) at the 

wavelength of 409 nm, and spectrophotometric determi-

nation of the amount of procyanidins in terms of cyanidin 

chloride (not less than 5.0%) at the wavelength of 550 

nm [28].

In our opinion, the conclusions regarding the objec-

tivity of this methodology can be made only after a deep 

study of the chemical composition of this type of raw 

materials.