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Table 1 – Retention time of the main peaks for the reference solution

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Морозов Проблемы хим стандарт

Table 2 – Retention time of the main peaks of the test solution

Table 1 – Retention time of the main peaks for the reference solution

Substance

Retention time, minutes

Chlorogenic acid

0.26

Hyperoside

1.4

Vitexin

1.0

Rutin

1.16

For the test solution, the retention time of the main

peaks consists of retention time for chlorogenic acid,

vitexin, rutin and hyperoside corresponding to that for

the reference solution, as well as the retention time of

acetylvitexin-2

-O-rhamnoside, vitexin, isovitexine and

vitexin-2

-O-rhamnoside, as shown in Table 2 [27].

113

Pharmacy & Pharmacology V. 6 N 2, 2018

DOI:10.19163/2307-9266-2018-6-2-104-120

Table 2 – Retention time of the main peaks of the test solution

Substance

Retention time, minutes

Acetylvinixine-2′′-O-rhamnoside

1.53

Vitexin

1.0

Izovitexin 0.73

Vitexin-2′′-O-rhamnoside

0.67

but not all the standard substances (rutin, chlorogenic

acid, hyperoside and vitexin) described in the US

Pharmacopeia for determining the authenticity of the

leaves with hawthorn fl owers, are specifi c parts of the

plant.

In the Belarusian Pharmacopoeia the section

“Authenticity” for hawthorn leaves also needs

improvement, since only qualitative reactions are

insuffi cient to identify the authenticity of raw materials.

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