Issn 305-9397. Ғылым және білім. 2022. №1-1 (66) issn 2305-9397
Ауыл шаруашылығы ғылымдары
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| ______ ____________Ауыл шаруашылығы ғылымдары
174 70-90V for 40 minutes. a) 1 – sample 81(2); 2 – sample 82-2(1); 3 – sample 82-2; 4 – sample 82-2(2); 5 – sample 82-2(2); 6 – sample 94-1; 7 – sample 95-2; 8 - sample 96; 9 - sample 97; M – 1 Kb Plus DNA Ladder molecular weight marker. b)1 – sample 1; 2 – sample 2; 3 – sample 5; 4 – sample 7; 5 – sample 8; 6 – sample 9; 7 – sample 12; 8 – sample 14; 9 - sample 15; M – 1 Kb Plus DNA Ladder molecular weight marker. Figure 1 – Electrophoregram: identification of bacterial DNA using the molecular marker 16S (a) and representatives of the genus Pseudomonas using the molecular marker rpoD (b) c)1 – sample 1-1; 2 – sample 1-3; 3 – sample 1-5; 4 – sample 1-6; 5 – sample 3-1; 6 – sample 3-2; 7 – sample 3-5; 8 – sample 3-6; 9 – sample 5-2; M – 1 Kb Plus DNA Ladder molecular weight marker. d)1 – sample 6-1; 2 – sample 6-2; 3 – sample 6-3; 4 – sample 6-4; 5 – sample 6-5; 6 – sample 6-6; 7 – sample 6-7; 8 – sample 6-8; 9 – sample 6-9; M – 1 Kb Plus DNA Ladder molecular weight marker. Figure 2 – Electrophoregram: identification of bacterial DNA of representatives of the genus Pseudomonas using the molecular marker gyrB (c) and genus Xanthomonas using the molecular marker fyuA (lanes 6-9) (d) e)1 – sample 7-1; 2 – sample 7-2; 3 – sample 7-3; 4 – sample 7-4; 5 – sample 7-5; 6 – sample 7-6; 7 – sample 7-7; 8 – sample 7-8; 9 – sample 7-9; M – 1 Kb Plus DNA Ladder molecular weight marker. f)1 – sample 8-1; 2 – sample 8-2; 3 – sample 8-3; 4 – sample 8-4; 5 – sample 8-5; 6 – sample 8-6; 7 – sample 8-7; 8 – sample 8-8; 9 – sample 8-9; 10 – sample 8-10; 11 – sample 8-11; 12 – sample 8-12; 13 – sample 8-13; 14 – sample 8-14; M – 1 Kb Plus DNA Ladder molecular weight marker. Figure 3 – Electrophoregram: identification of bacterial DNA of representatives of the genus Xanthomonas using the molecular marker rpoD (e) and genus Pseudomonas using the molecular marker gltA (f) Thus, ecological and genetic monitoring of pathogens of the most dangerous bacterial diseases of wheat and other cereal crops was carried out - pathogens of black (Xanthomonas campestris) and basal (Pseudomonas syringae) bacterioses of wild and agricultural cereal crops. Samples of wild and agricultural cereal crops were collected in the north of Kazakhstan, and bacterioses infecting cereal |