Issn 305-9397. Ғылым және білім. 2022. №1-1 (66) issn 2305-9397


Results and its discussion



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Журнал Наука и образование №3-3(68) 2022

Results and its discussion. The most important limit in the production of meat products is 
the low initial contamination (hygiene) of meat raw materials. The change of microflora begins at the 
time of animal slaughter and can be exogenous (by taking microflora from the external environment - 
from the surface of the skin, tools, equipment, hands of employees, air of industrial premises).
According to the requirements of TR KO "On the safety of meat and meat products" (TR KO 
034/2013), the total contamination of raw meat on the bone should not exceed 10
3
CFU/g. But the 
shelf life of the manufactured product is directly related to the high requirements for the primary 
contamination of meat raw materials. 
In the work, the number of microorganisms with the process of receiving raw materials was 
studied (Table 1). 
The main groups of microorganisms in tendons during raw intake were determined. In terms 
of numerical composition, the largest group of mesophilic aerobic and facultatively anaerobic 
microorganisms, yeasts and molds, and spore-forming bacteria was found. 
Table 1 – Indicators of initial contamination of collagen raw materials with microorganisms 
From the data in table 1, as a result of the initial contamination indicators of collagen-
containing raw materials with microorganisms, MAzhFAMs 2.1 * 10
3
, Yeasts 17, mold fungi 24, 
spore-forming bacteria 24 CFU/g showed indicators, that is, the number of sanitary microorganisms 
"On the safety of meat and meat products" TR KO showed that it does not meet the requirements (TR 
KO 034/2013). 
During heat treatment up to 68 ... 72 °C, up to 99% of microorganisms can be killed, but since 
the collagen hydrolyzation technology is based on enzymes, the hydrolysis temperature does not 
exceed 40 °C so that the protein does not undergo denaturation. 
Thus, a low rate of contamination by microorganisms is the first and most important criterion 
that determines the guaranteed level of safety and quality, the possible duration of storage (shelf life), 
and ensuring safety criteria in production conditions is the main task of technologists. 
Before hydrolysis, it is recommended to disinfect raw materials, because during the mild 
fermentation process favorable conditions are created for the growth of microorganisms, their number 
increases by 20-30%. 
Spice extracts, lysozyme, chitosan, lactoferrin, pectin hydrolyzate, protamine, paprika 
glycoprotein, hop extracts, etc., are used to stop the growth of meat microorganisms [16]. 
Microorganisms name, CFU/g 
Microorganisms 
indicator, 
CFU/g 
Normal indicator 
according to the 
technical regulation of 
TR 021/2011 of the 
Central Committee on 
food safety 
Аmount of mesophilic aerobic and facultative 
anaerobic microorganisms 1*103 
2.1 

Сoli group bacteria 
Not identified 
Not identified 
Sulfide reducing microorganisms 
Not identified 
Not allowed 
S. aureus 0.1 g 
Not identified 
Not allowed 
Spore-forming microorganisms 1 *103 


Pathogenic microorganisms 
Not identified 
Not allowed 
Mold fungi 
24 
Not allowed 
Yeasts 
17 
10 




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