Issn 305-9397. Ғылым және білім. 2022. №1-1 (66) issn 2305-9397
ISSN 2305-9397. Ғылым және білім. 2022. № 3-3 (68)
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| ISSN 2305-9397. Ғылым және білім. 2022. № 3-3 (68)____ _
175 crops were identified using ITS sequences of the DNA region. Conclusions. Ecological and genetic monitoring of pathogens of the most dangerous bacterial diseases of wheat and other cereal crops was carried out - pathogens of black (Xanthomonas campestris) and basal (Pseudomonas syringae) bacterioses of wild and agricultural cereal crops. In total, 93 plant samples of cereal crops were collected in the west and north of Kazakhstan with symptoms of bacterial diseases, and 120 DNA samples were isolated. Molecular screening of pathogens of bacterial diseases using specific markers shows the presence of pathogens of basal and black bacteriosis and their pathovars in the northern region of Kazakhstan. Bacterioses infecting cereal crops were identified using ITS region sequences. The causative agent of basal bacteriosis (Pseudomonas syringae) was identified in samples from the Karaganda, Kostanay, and Akmola regions, which is confirmed by the results of sequencing of the species-specific genome region of this bacterium. Funding. This study was funded by the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan (grant No. OR11465424). REFERENCES 1 Zheldakova R.A., Myamin V.E. Fitopatogennye mikroorganizmy: Ucheb.- metod. kompleks dlya studentov boil. Fak. Spec. G – 31 01 01 «Biologiya» / R. A. Zheldakova, V.E. Myamin. ˗ Mn. : BGU, 2006. ˗ 116 с. 2 Duveiller E., Singh P.K., Meccalama M., Singh R.P., Dababat A. Bolezni i vrediteli pshenicy. / ˗ Ankara, ˗ 2018. ˗ 160 с. 3 Xin X. F., Kvitko B., He S. Y. Pseudomonas syringae: what it takes to be a pathogen //Nature Reviews Microbiology. – 2018. – Т. 16. – № 5. – С. 316-328. 4 Kazempour M.N, Kheyrgoo M., Pedramfar H., Rahimian H., Isolation and identification of bacterial glum blotch and leaf blight on wheat (Triticum aestivum L.) in Iran. African Journal of Biotechnology. – 2010. – Т.9. ˗ № 20. – С. 2860-2865. 5 Pseudomonas syringae pv. syringae. [Distribution map]. 4 September 2008 CAB International Wallingford UK. DOI.10.1079/DMPD/20046500336. 6 Sands D.C., Fourrest E., Xanthomonas campestris pv. translucens in North and South America and in the Middle East // EPPO Conference on Bacterial Diseases of Gramineae, Paderborn (DE). – 1988. – Т. 19. ˗№ 1. – С. 127-130. DOI.org/10.1111/j.1365-2338.1989.tb00138.x 7 Ryan R. P. et al. Pathogenomics of Xanthomonas: understanding bacterium–plant interactions //Nature Reviews Microbiology. – 2011. – Т. 9. – №. 5. – P. 344-355. 8 Koishybaev M. Bolezni pshenicy. – Prodovolstvennaya i selskohozyaistvennaya organizaciya OON (FAO) / Ankara, ˗ 2018. – 394 st. 9 Tambong J.T., Xu R., Gerdis S., Daniels G.C., Chabot D., Hubbard K., Harding M.W. Molecular analysis of bacterial isolates from necrotic wheat leaf lesions caused by Xanthomonas translucens, and description of three putative novel species, Sphingomonas albertensis sp. nov., Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov. Front. Microbiol. – 2021. – Т. 12:666689. DOI.org/10.3389/fmicb.2021.666689 10 Zaharova N.G., Vershinina V.I., Il'inskaya O.N. Kratkii kurs po mikrobiologii, virusologii i immunologii N.G. Zaharova, V.I. Vershinina, O.N. Ilinskaya / – Kazan: 2015. – 799 st. 11 Borrelli V.M.G., Brambilla V., Rogowsky P., Marocco A., Lanubile A. The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology // Front. Plant Sci. – 2018. – Т. 9:1245. doi: 10.3389/fpls.2018.01245. 12 Tyagi S., Kumar R., Kumar V., Won S.Y., Shukla P. Engineering disease resistant plants through CRISPR-Cas9 technology // GM Crops & Food. – 2021. – Т. 12. ˗ №1. – P. 125-144. DOI: 10.1080/21645698.2020.1831729. 13 Tambong J.T., Xu R., Gerdis S., Daniels G.C., Chabot D., Hubbard K., Harding M.W. Molecular Analysis of Bacterial Isolates From Necrotic Wheat Leaf Lesions Caused by Xanthomonas translucens, and Description of Three Putative Novel Species, Sphingomonas albertensis sp. nov., Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov. // Front. Microbiol. – 2021. – Т. 12:666689. doi: 10.3389/fmicb.2021.666689. |